|
KMID : 0606920110190040451
|
|
Biomolecules & Therapeutics
2011 Volume.19 No. 4 p.451 ~ p.459
|
|
|
Over-expressed Peroxiredoxin I Protects against Oxidative Damage in Mouse Embryonic Fibroblasts Lacking Peroxiredoxin II
|
|
Kim Seong-Gon
Kim Jae-Young
Ryoo Zae-Young
Lee Sang-Gyu
|
|
|
Abstract
|
|
|
|
Peroxiredoxins (Prxs) have a critical role in protecting cells against oxidative damage generated by reactive oxygen species (ROS). PrxI and PrxII are more than 90% homologous in their amino acid sequences, and both proteins reduce H2O2. In this study, an over-expression plasmid carrying PrxI was transfected into PrxII?/? mouse embryonic fi broblasts (MEFs) to investigate potential compensatory relationships between PrxI and PrxII. ROS levels induced by oxidative stress were increased in PrxII?/? MEFs as compared to wild-type MEFs. Moreover, exposure of PrxII?/? MEFs to H2O2 caused a reduction in cell viability of about 10%, and the proportion of cell death was increased compared to mock-treated PrxII?/? MEFs. However, transient over-expression of PrxI in PrxII?/? MEFs conferred increased resistance against the oxidative damage, as evidenced by increased cell viability and reduced intracellular ROS levels under H2O2 stress conditions. The fi ndings suggest that over-expressed PrxI can partly compensate for the loss of PrxII function in PrxII-defi cient MEFs.
|
|
|
KEYWORD
|
|
|
Peroxiredoxin I, Peroxiredoxin II, ROS, MEF
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|